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| Saturday, September 27th , 2025 |
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| Fort Myers Miracle: Activated LF Raúl Molina from the injured list. |
| Fort Myers Miracle: Activated RF Joe Collins from the injured list. |
| Thursday, September 25th , 2025 |
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| Charlotte Stone Crabs: RF Jeff Viitaluoma retired from professional baseball. |
| Daytona Tortugas: LF Yeong-seon Lee retired from professional baseball. |
| Charlotte Stone Crabs: RP Guadalupe Chavez retired from professional baseball. |
| Dunedin Blue Jays: RP Kevin McGhee retired from professional baseball. |
| Daytona Tortugas: RP Bob Dillon retired from professional baseball. |
| St. Lucie Mets: C Bob Williams retired from professional baseball. |
| Bradenton Marauders: CF Dave Sherman retired from professional baseball. |
| Lakeland Flying Tigers: CF Dave Murphy retired from professional baseball. |
| Bradenton Marauders: RP Joe Wolfe retired from professional baseball. |
| St. Lucie Mets: CF Luis Carmona retired from professional baseball. |
| Clearwater Threshers: 2B Chad Starr retired from professional baseball. |
| Charlotte Stone Crabs: RP Lou Woods retired from professional baseball. |
| St. Lucie Mets: SS Alan Lucas retired from professional baseball. |
| Daytona Tortugas: 3B Jeff Stewart retired from professional baseball. |
| Daytona Tortugas: LF Vince Rabe retired from professional baseball. |
| Charlotte Stone Crabs: RP Don Hart retired from professional baseball. |
| St. Lucie Mets: 1B Brad Oglethorpe retired from professional baseball. |
| Lakeland Flying Tigers: C Pat Christenson retired from professional baseball. |
| Lakeland Flying Tigers: RP Jeremy Harvey retired from professional baseball. |
| St. Lucie Mets: RP Eric Webster retired from professional baseball. |
| Dunedin Blue Jays: 2B Cal Bocanegra retired from professional baseball. |
| Fort Myers Miracle: RP Ken Butler retired from professional baseball. |
| Bradenton Marauders: C Bob Thompson retired from professional baseball. |
| Fort Myers Miracle: RP Steve Gray retired from professional baseball. |
| St. Lucie Mets: RP Darryl Stone retired from professional baseball. |
| Bradenton Marauders: C Dave Weeks retired from professional baseball. |
| Palm Beach Cardinals: RP Wing-chiu Huang retired from professional baseball. |
| Lakeland Flying Tigers: RP Enzo Garin retired from professional baseball. |
| Dunedin Blue Jays: LF Willie Hawkins retired from professional baseball. |
| Clearwater Threshers: RP Steve Black retired from professional baseball. |
| Clearwater Threshers: C Oscar Stanton retired from professional baseball. |
| Jupiter Hammerheads: SP Bobby Harris retired from professional baseball. |
